Year: 2014 | Month: September | Volume 7 | Issue 3
Biochemical Characterization and Molecular Fingerprinting of Plant Growth Promoting Rhizobacteria
Abstract:
Plant growth promoting rhizobacteria (PGPR) are a heterogeneous group of bacteria that can be found in the rhizosphere, which can improve the extent or quality of plant growth directly or indirectly. However, screening strategies for selecting the best rhizobacteria strain for rhizosphere competence with other microbial species in the plant rhizosphere will require more comprehensive knowledge. In the present investigation nine different strains were tested for their PGPR properties by using RFLP analysis on 16S rRNA gene or amplified rDNA restriction analysis (ARDRA). 16 S rDNA amplification was done and restriction profiling was done using two endonucleases i.e. msp1 and taq1. Depending upon banding pattern of all the nine strains dendrogram was created using NTsys software. A clear-cut difference was seen in genetic diversity among the strains. Pseudomonas was found to be the most effective strain among all. Depending upon the outcome we can conclude that ARDRA can be an effective tool for analyzing the genetic diversity among different bacteria and PGPR starin e.g. Pseudomonas, Bacillus can be used as a potent biofertilizer.
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